Fluorescent Nucleic Acid Hybridization Probes
With the introduction of simple and relatively inexpensive methods for labeling nucleic acids with non-radioactive labels, doors have been opened that enable nucleic acid hybridization probes to be used for research and development, as well as for clinical diagnostic applications.
Below is an overview of the different types of fluorescent nucleic acid hybridization probes that have been introduced. Although the mechanism of fluorescence generation is different among the different types of fluorescent nucleic acid hybridization probes, they all are labeled with at least one molecule, a fluorophore, that has the ability to absorb energy from light, transfer this energy internally, and emit this energy as light of a characteristic wavelength.
Molecular Beacon Probes
Wavelength-Shifting Molecular Beacon Probes
Bi-Molecular Beacon Probes
Tripartite Molecular Beacon Probes
Dumbbell Molecular Beacon Probes
Minor Groove Binder (MGB) Probes
Strand Displacement Probes
Duplex Scorpion Primers