Fluorescent Nucleic Acid Hybridization Probes

With the introduction of simple and relatively inexpensive methods for labeling nucleic acids with non-radioactive labels, doors have been opened that enable nucleic acid hybridization probes to be used for research and development, as well as for clinical diagnostic applications.

Below is an overview of the different types of fluorescent nucleic acid hybridization probes that have been introduced. Although the mechanism of fluorescence generation is different among the different types of fluorescent nucleic acid hybridization probes, they all are labeled with at least one molecule, a fluorophore, that has the ability to absorb energy from light, transfer this energy internally, and emit this energy as light of a characteristic wavelength.

Molecular Beacon Probes


Wavelength-Shifting Molecular Beacon Probes


Bi-Molecular Beacon Probes


Tripartite Molecular Beacon Probes


Molecular Torches


Tentacle Probes


Amplifluor Primers


Scorpion Primers


Dumbbell Molecular Beacon Probes




Taqman Probes


Minor Groove Binder (MGB) Probes




Adjacent Probes


Strand Displacement Probes


Duplex Scorpion Primers


Qual Probes


Light-Up Probes